Conditioned medium from rat dental pulp reduces the number of osteoclasts via attenuation of adhesiveness in osteoclast precursors
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- Mori Hironori
- Department of Pharmacology, School of Dentistry, Aichi Gakuin University Department of Orthodontics, School of Dentistry, Aichi Gakuin University
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- Hamamura Kazunori
- Department of Pharmacology, School of Dentistry, Aichi Gakuin University
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- Yo Shoyoku
- Department of Pharmacology, School of Dentistry, Aichi Gakuin University Department of Orthodontics, School of Dentistry, Aichi Gakuin University
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- Hamajima Kosuke
- Department of Pharmacology, School of Dentistry, Aichi Gakuin University Department of Orthodontics, School of Dentistry, Aichi Gakuin University
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- Ootani Kenji
- Advanced Cell Technology and Engineering Ltd
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- Honda Masaki
- Department of Oral Anatomy, School of Dentistry, Aichi Gakuin University
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- Ishizuka Kyoko
- Department of Pharmacology, School of Dentistry, Aichi Gakuin University
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- Kondo Hisataka
- Department of Pharmacology, School of Dentistry, Aichi Gakuin University
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- Tanaka Kenjiro
- Department of Pharmacology, School of Dentistry, Aichi Gakuin University
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- Kodama Daisuke
- Laboratory of Neuropharmacology, School of Pharmacy, Aichi Gakuin University
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- Hirai Takao
- Laboratory of Medical Resources, School of Pharmacy, Aichi Gakuin University
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- Miyazawa Ken
- Department of Orthodontics, School of Dentistry, Aichi Gakuin University
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- Goto Shigemi
- Department of Orthodontics, School of Dentistry, Aichi Gakuin University
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- Togari Akifumi
- Department of Pharmacology, School of Dentistry, Aichi Gakuin University
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説明
<p>Dental pulp is known to play crucial roles in homeostasis of teeth and periodontal tissue. Although resorption of bone around the roots of nonvital teeth is occasionally observed in clinical practice, little is known about the role of dental pulp in osteoclastogenesis. Here we evaluated the effects of conditioned medium (CM) from rat dental pulp on osteoclastogenesis. It was found that the CM reduced the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated osteoclasts, but did not alter the mRNA levels of nuclear factor of activated T-cells, cytoplasmic 1 and TRAP. To further understand the mechanism behind these results, we evaluated the effects of CM on osteoclast precursors and found that the CM removed cell processes, resulting in a significant reduction in the number of attached cells and an increase in the number of freely floating cells. Furthermore, the CM suppressed the mRNA levels of focal adhesion kinase and paxillin, which are involved in cell adhesiveness and spreading. Collectively, the present results show that CM from dental pulp serves as an inhibitor of osteoclastogenesis by reducing the number and adhesiveness of osteoclast precursors, suggesting novel therapeutic applicability for osteoporosis.</p>
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ
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Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ 60 (3), 352-359, 2018
日本大学歯学部