Profiling of bryophyte gene expression by hybridization of an Arabidopsis cDNA array with bryophyte cDNA
-
- JIN CHUNG SUNG
- Graduate School of Science and Technology, Kumamoto University
-
- TAKECHI KATSUAKI
- Faculty of Science, Kumamoto University
-
- SAKAI ATSUSHI
- Faculty of Science, Nara Women's University
-
- ONO KANJI
- Faculty of Science, Kumamoto University
-
- TAKANO HIROYOSHI
- Faculty of Science, Kumamoto University
書誌事項
- タイトル別名
-
- PROFILING OF BRYOPHYTE GENE EXPRESSION BY HYBRIDIZATION OF AN <i>ARABIDOPSIS</i> cDNA ARRAY WITH BRYOPHYTE cDNA
この論文をさがす
抄録
<p> Heterologous hybridization was carried out with an Arabidopsis macroarray and cDNAs synthesized from total RNAs of the liverwort Marchantia polymorpha and the moss Physcomitrella patens. Total RNA isolated from A. thaliana plants was also used. The macroarray contained 5,760 Arabidopsis ESTs, corresponding to 4,372 genes. Intra- and inter-filter variations showed less than 2-fold range for almost all of the spots. Genes numbering 1,647 (37.7%), 1,427 (32.6%), and 1,217 (27.8%) had hybridization signals with intensities three-fold greater than that of λ DNA (negative control), and were thus defined as expressed in A. thaliana plants, M. polymorpha thalli, and P. patens protonemata, respectively. Seventy-nine percent of the genes expressed in M. polymorpha were also expressed in P. patens. Overall, the three species had 763 expressed genes in common. Twenty-five co-expressed genes were chosen, based on their high expression levels in M. polymorpha, and 17 in P. patens EST clones related to these genes were identified, each showing more than 60% identity with the corresponding A. thaliana gene at the nucleotide level. Three hundred and sixty three genes were detected in bryophytes but not in A. thaliana. Of the 25 highly expressed bryophyte-specific genes, 14 had the P. patens EST homologs with greater than 60% identity to an A. thaliana gene. These results suggest that hybridization of Arabidopsis macroarrays with heterologous cDNA is a useful tool for gene expression profiling of distantly related plant species such as bryophytes.</p>
収録刊行物
-
- 財団法人服部植物研究所報告
-
財団法人服部植物研究所報告 99 (0), 233-244, 2006-01-20
公益財団法人 服部植物研究所
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001288072384512
-
- NII論文ID
- 130007484907
- 40007091458
-
- NII書誌ID
- AA10665989
-
- ISSN
- 24328944
- 00730912
-
- NDL書誌ID
- 7783326
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可