Hypotaurine Is a Substrate of GABA Transporter Family Members GAT2/Slc6a13 and TAUT/Slc6a6
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- Nishimura Tomohiro
- Faculty of Pharmacy, Keio University
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- Higuchi Kei
- Faculty of Pharmacy, Keio University Faculty of Pharma-Sciences, Teikyo University
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- Yoshida Yuko
- Faculty of Pharmacy, Keio University
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- Sugita-Fujisawa Yuki
- Faculty of Pharmacy, Keio University
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- Kojima Kazuaki
- Faculty of Pharmacy, Keio University
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- Sugimoto Maiko
- Faculty of Pharmacy, Keio University
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- Santo Marie
- Faculty of Pharmacy, Keio University
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- Tomi Masatoshi
- Faculty of Pharmacy, Keio University
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- Nakashima Emi
- Faculty of Pharmacy, Keio University
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抄録
<p>Hypotaurine is a precursor of taurine and a physiological antioxidant that circulates in adult and fetal plasma. The purpose of the present study was to clarify whether hypotaurine is a substrate of Slc6a/gamma-aminobutyric acid (GABA) transporter family members. Radiolabeled hypotaurine was synthesized from radiolabeled cysteamine and 2-aminoethanethiol dioxygenase. The uptakes of [3H]GABA, [3H]taurine, and [14C]hypotaurine by HEK293 cells expressing mouse GAT1/Slc6a1, TAUT/Slc6a6, GAT3/Slc6a11, BGT1/Slc6a12, and GAT2/Slc6a13 were measured. TAUT and GAT2 showed strong [14C]hypotaurine uptake activity, while BGT1 showed moderate activity, and GAT1 and GAT3 showed slight but significant activity. Mouse TAUT and GAT2 both showed Michaelis constants of 11 µM for hypotaurine uptake. GAT2-expressing cells pretreated with hypotaurine showed resistance to H2O2-induced oxidative stress. These results suggest that under physiological conditions, TAUT and GAT2 would be major contributors to hypotaurine transfer across the plasma membrane, and that uptake of hypotaurine via GAT2 contributes to the cellular resistance to oxidative stress.</p>
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 41 (10), 1523-1529, 2018-10-01
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001288073615616
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- NII論文ID
- 130007491801
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 029238490
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- PubMed
- 30270321
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
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