Screening of Heat-Resistant Bacteria Form Fruit Canning Factory by EMA-PCR-DGGE

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  • EMA-PCR-DGGE法によるフルーツ缶詰製造工場からの耐熱性菌探索
  • EMA-PCR-DGGEホウ ニ ヨル フルーツ カンズメ セイゾウ コウジョウ カラ ノ タイネツセイキン タンサク

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<p>Heat-resistant microorganisms can cause food deterioration even after heat sterilization. Generally, heat-resistant microorganisms are screened by cultivating heat-treated samples. However, biased cultivation conditions can inhibit comprehensive isolation of these microorganisms. EMA-PCR-DGGE is considered a comprehensive detection method to selectively analyze viable microbial flora that eliminates bias in the cultivation conditions.</p><p>In this study, we screened heat-resistant bacteria by analyzing heated samples using EMA-PCR-DGGE. Specifically, the screening was performed on wipe test fluid samples from a fruit canning factory, and the bacteria were isolated by the addition of cultivation conditions on the screened strains.</p><p>We successfully isolated Micromonospora sp. and Propionibacterium sp. The evaluation of heat resistance in these bacteria revealed that Micromonospora sp. was indeed heat resistant; thus, the validity of this method for screening heat-resistant bacteria was confirmed.</p>

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