Recovery Effects of Trehalose on Acid Denaturation/Aggregation of Proteins

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  • トレハロースによるタンパク質の酸変性及び凝集に対する回復作用
  • トレハロース ニ ヨル タンパクシツ ノ サン ヘンセイ オヨビ ギョウシュウ ニ タイスル カイフク サヨウ

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Abstract

<p>Osmolytes, including sugars and polyols, are well known to suppress denaturation and aggregation of proteins and prevent deactivation of enzymes. Trehalose, a nonreducing disaccharide, is thought to have an excellent stabilizing action. Trehalose is produced by organisms with tolerance to extreme environments. By using synchrotron radiation X-ray wide-angle scattering (WAXS), we have found that trehalose dissociates aggregates of bovine myoglobin denatured by acidic solvents, and then folds it into a native structure. Denaturants and surfactants have been used as additives that dissociate aggregates; however, most of them are cytotoxic. Therefore, this report would suggest that trehalose is a promising candidate as a protein aggregation dissociator that is harmless to the human body. In addition, this study shows the usefulness of the wide-angle scattering method for protein structure analysis in the presence of co-solute.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 68 (1), 43-49, 2019-01-05

    The Japan Society for Analytical Chemistry

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