Off- and on-target effects of genome editing in mouse embryos
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- AYABE Shinya
- Experimental Animal Division, RIKEN BioResource Research Center, Ibaraki 305-0074, Japan
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- NAKASHIMA Kenichi
- Gene Engineering Division, RIKEN BioResource Research Center, Ibaraki 305-0074, Japan
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- YOSHIKI Atsushi
- Experimental Animal Division, RIKEN BioResource Research Center, Ibaraki 305-0074, Japan
Bibliographic Information
- Other Title
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- Opinions and Hypotheses : Off- and on-target effects of genome editing in mouse embryos
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Abstract
<p> Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome. Unbiased whole genome sequencing comparing parents to progeny has revealed that the rate of Cas9-induced mutagenesis in mouse embryos is indistinguishable from the background rate of de novo mutation. However, establishing the best practice to confirm on-target alleles of interest remains a challenge. We believe that improvement in editing strategies and screening methods for founder mice will contribute to the generation of quality-controlled animals, thereby ensuring reproducibility of results in animal studies and advancing the 3Rs (replacement, reduction, and refinement).</p>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 65 (1), 1-5, 2019
The Society for Reproduction and Development
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Keywords
Details 詳細情報について
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- CRID
- 1390001288122173184
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- NII Article ID
- 130007590994
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 029510788
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- PubMed
- 30518723
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed