CCL20 Promotes Ovarian Cancer Chemotherapy Resistance by Regulating ABCB1 Expression

DOI Web Site Web Site PubMed 29 References
  • Su Shan
    Department of Gynecology, the Central Hospital of Zibo
  • Sun Xueqin
    Department of Gynecology, the Central Hospital of Zibo
  • Zhang Qinghua
    Department of Gynecology, the Central Hospital of Zibo
  • Zhang Zhe
    Department of Gynecology, the Central Hospital of Zibo
  • Chen Ju
    Department of Ultrasound, the Central Hospital of Zibo

Search this article

Description

<p>Ovarian cancer (OC) is one of prevalent tumors and this study aimed to explore CCL20’s effects on doxorubicin resistance of OC and related mechanisms. Doxorubicin-resistant SKOV3 DR cells were established from SKOV3 cells via 6-month continuous exposure to gradient concentrations of doxorubicin. Quantitative PCR and Western blot assay showed that SKOV3 DR cells had higher level of CCL20 than SKOV3 cells, and doxorubicin upregulated CCL20 expression in SKOV3 cells. MTT and cell count assay found that CCL20 overexpression plasmid enhanced doxorubicin resistance of SKOV3 and OVCA433 cells compared to empty vector, as shown by the increase in cell viability. In contrast, CCL20 shRNA enhanced doxorubicin sensitivity of SKOV3 DR cells compared to control. CCL20 overexpression plasmid promoted NF-kB activation and positively regulated ABCB1 expression. Besides, ABCB1 overexpression plasmid enhanced the viability of SKOV3 and OVCA433 cells compared to empty vector under treatment with the same concentration of doxorubicin, whereas ABCB1 shRNA inhibited doxorubicin resistance of SKOV3 DR cells compared to control. In conclusion, CCL20 enhanced doxorubicin resistance of OC cells by regulating ABCB1 expression.</p><p>Key words: CCL20, ovarian cancer, doxorubicin resistance, tumor-promoting, ABCB1</p>

Journal

References(29)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top