Development of highly sensitive enzyme immunoassay （ICT-EIA） for IA-2 autoantibody and application for detection of three islet-specific autoantibodies （ GADA, IA-2A and IAA） in type I diabetes mellitus

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  • Kawano Tomoharu
    Student,Doctorate Course in Food Sciences ,Graduate School of Human Life Siences ,Tokushima Bunri University
  • Numata Satoshi
    Kochi Prefectural University College of Health and Nutrition
  • Fujimoto Yuki
    Health Science　Institute,Tokushima Bunri University
  • Kuroda Akio
    Diabetes Therapeutics and Research Center, Institute of Advanced Medical Sciences, Tokushima University
  • Yasuda Tetsuyuki
    Department of Diabetes and Endocrinology, Osaka Police Hospital
  • Miyashita Kazuyuki
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University
  • Sakamoto Fumie
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University
  • Katakami Naoto
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University
  • Matsuoka Taka-aki
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University
  • Matsuhisa Munehide
    Diabetes Therapeutics and Research Center, Institute of Advanced Medical Sciences, Tokushima University
  • Hashida Seiichi
    Health Science　Institute,Tokushima Bunri University

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Other Title
  • IA-2 抗体の高感度検出法（ICT-EIA 法）の開発と 長期罹病若年性1 型糖尿病患者における 3 種膵島関連自己抗体（GADA<b>，</b>IA-2A<b>，</b>IAA）の検出について
  • IA-2抗体の高感度検出法(ICT-EIA法)の開発と長期罹病若年性1型糖尿病患者における3種膵島関連自己抗体(GADA, IA-2A, IAA)の検出について
  • IA-2 コウタイ ノ コウカンド ケンシュツホウ(ICT-EIAホウ)ノ カイハツ ト チョウキリビョウ ジャクネンセイ 1ガタ トウニョウビョウ カンジャ ニ オケル 3シュスイトウ カンレン ジコ コウタイ(GADA, IA-2A, IAA)ノ ケンシュツ ニ ツイテ

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<p>Development of highly sensitive enzyme immunoassay （ICT-EIA） for IA-2 autoantibody and application for detection of three islet-specific autoantibodies （ GADA, IA-2A and IAA） in type I diabetes mellitus Tomoharu Kawano, Satoshi Numata, Yuki Fujimoto, Akio Kuroda, Tetsuyuki Yasuda,Kazuyuki Miyashita, Fumie Sakamoto, Naoto Katakami, Taka-aki Matsuoka, Munehide Matsuhisa and Seiichi Hashida Summary Detection of pancreatic islet-related autoantibodies（ insulin antibody [IAA]; glutamic acid decarboxylase antibody [GADA]; and islet antigen-2 antibody [IA-2A], is essential for the diagnosis and prediction of the onset of type I diabetes mellitus. Depending on the disease duration, the detection rate of each autoantibody is different, and using only one kind of autoantibody detection risks overlooking a positive result. The detection precision of type I diabetes mellitus would thus be improved by measuring all three kinds of autoantibodies. We developed a highly sensitive enzyme immunoassay（ ICT-EIA） for IA-2 autoantibody. ICT-EIAs for IAA and GADA have already been developed. Using three ICT-EIAs, we attempted to measure autoantibodies in sera from patients with type I diabetes mellitus. Patients comprised 25 males and 51 females（ mean age, 33.3±5.7 years; disease duration, 23.9±6.7 years） with type I diabetes mellitus who had been undergoing treatment with insulin for between 11 and 40 years. Among these patients, urinary C-peptide-positive results were seen in three patients. IAA, GADA and IA-2A in sera were measured by ICT-EIA. The newly developed ICT-EIA for IA-2 antibodies was approximately 100-times more sensitive than the conventional ELISA. The positive ratio was 77.6%, representing a high rate of detection. In addition, even in patients with a disease duration >30 years, the positive rate was 64.3%. ICT-EIA was thus suggested to be useful in combination assays for pancreatic islet-related autoantibodies （IAA, GADA and IA-2A）.</p><p> </p><p></p>

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