Isolation of Sphingoid Bases from Starfish Asterias amurensis Glucosylceramides and Their Effects on Sphingolipid Production in Cultured Keratinocytes
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- Mikami Daisuke
- Laboratory of Biomembrane and Biofunctional Chemistry, Frontier Research Center for Advanced Material and Life science, Hokkaido University
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- Sakai Shota
- Laboratory of Biomembrane and Biofunctional Chemistry, Frontier Research Center for Advanced Material and Life science, Hokkaido University Department of Biochemistry & Cell Biology, National Institute of Infectious Diseases
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- Yuyama Kohei
- Laboratory of Biomembrane and Biofunctional Chemistry, Frontier Research Center for Advanced Material and Life science, Hokkaido University
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- Igarashi Yasuyuki
- Laboratory of Biomembrane and Biofunctional Chemistry, Frontier Research Center for Advanced Material and Life science, Hokkaido University
書誌事項
- タイトル別名
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- Isolation of Sphingoid Bases from Starfish <i>Asterias amurensis</i> Glucosylceramides and Their Effects on Sphingolipid Production in Cultured Keratinocytes
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説明
<p>Starfish Asterias amurensis produces sphingoid bases d18:3, 9-methyl-d18:3 (9Me-d18:3), and d22:2, which possess unique structural features. In this study, sphingoid bases prepared from A. amurensis glucosylceramides displayed unexpected elution behaviors from a general octadecyl silyl high-performance liquid chromatography (HPLC) column. For separation and isolation, sphingoid bases were fractionated by octadecyl silyl HPLC after N-acetylation, yielding d18:3, 9Me-d18:3, and two d22:2 isomers. To compare the biological activities of individual sphingoid bases, their effects on sphingolipid production in normal human keratinocytes were evaluated. Treatment with sphingoid bases increased the content of ceramides, glucosylceramides, and sphingomyelins in keratinocytes. Moreover, ceramides, which contain saturated ultra-long-chain fatty acids (C30–34), were significantly increased by treatment with d18:3, but not with other A. amurensis sphingoid bases. The mRNA level of the early differentiation marker keratin 10 was markedly decreased and sphingolipid synthesis-related genes were slightly increased in keratinocytes exposed to A. amurensis-derived d18:3, 9Me-d18:3, and d22:2 isomers. These results suggest that A. amurensis-derived sphingoid bases induce differentiation to varying degrees, sphingolipid production depends on their chemical structures, and d18:3 is the most promising functional sphingoid base.</p>
収録刊行物
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- Journal of Oleo Science
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Journal of Oleo Science 68 (5), 427-441, 2019
公益社団法人 日本油化学会
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詳細情報 詳細情報について
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- CRID
- 1390001288135095040
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- NII論文ID
- 130007641011
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- NII書誌ID
- AA11503337
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- ISSN
- 13473352
- 13458957
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- NDL書誌ID
- 029661027
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- PubMed
- 30971644
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可