Retinoic Acid‐inducible Gene‐I and CXCL10 are Involved in Biliary Atresia

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  • Kimura Toshiro
    Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital
  • Imaizumi Tadaatsu
    Department of Vascular Biology, Hirosaki University Graduate School of Medicine
  • Yoshida Tatsuya
    Department of Vascular Biology, Hirosaki University Graduate School of Medicine
  • Shimada Taku
    Department of Vascular Biology, Hirosaki University Graduate School of Medicine
  • Hayakari Ryo
    Department of Vascular Biology, Hirosaki University Graduate School of Medicine
  • Kawaguchi Shogo
    Department of Gastroenterology and Hematology, Hirosaki University Graduate School of Medicine
  • Yoshida Hidemi
    Department of Vascular Biology, Hirosaki University Graduate School of Medicine
  • Kobayashi Tamotsu
    Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital
  • Hirabayashi Takeshi
    Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital
  • Mizukami Hiroki
    Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine
  • Kijima Hiroshi
    Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine
  • Hakamada Kenichi
    Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital

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Other Title
  • Retinoic Acid-Inducible Gene-i and CXCL10 are Involved in Biliary Atresia

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Abstract

Purpose: Retinoic acid-inducible gene-I（RIG-I）is a member of cytoplasmic viral sensors which plays an important role in inflammation of biliary epithelial cells（BECs）. The aim of this study is to examine if RIG-I and C-X-C motif chemokine 10（CXCL10）are involved in the etiology of human biliary atresia（BA）.<br> Methods: Immunohistochemical study was performed on surgically resected tissues obtained（June 1994 to March 2016） from 30 infants with BA and non-inflamed hepatic tissues from 7 infants with hepatoblastoma. A semiquantitative scoring system was designed to evaluate the staining with an antibodies to the RIG-I and CXCL10. The expression of RIG-I and CXCL10 in HuCCT1 cholangiocarcinoma cell line were studied by western blotting, ELISA and RT-PCR analyses.<br> Results: Intense immunoreactivity for RIG-I and CXCL10 was detected in BECs in tissues resected from BA patients. The expression of RIG-I and CXCL10 in the hilar tissue was significantly stronger than in the hepatic tissue. Transfection of HuCCT1 cells with poly（I:C）, a synthetic analog of viral dsRNA, induced the expression of RIG-I, and knockdown of RIG-I inhibited the induction of CXCL10 in HuCCT1 cells transfected with poly（I:C）.<br> Conclusion: These results suggest that RIG-I-CXCL10 cascade may be involved in the etiology of human BA.

Journal

  • Hirosaki Medical Journal

    Hirosaki Medical Journal 69 (1-4), 86-94, 2019-03-15

    Hirosaki University Graduate School of Medicine,Hirosaki Medical Society

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