Retinoic Acid-Inducible Gene-i and CXCL10 are Involved in Biliary Atresia
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- Kimura Toshiro
- Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital
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- Imaizumi Tadaatsu
- Department of Vascular Biology, Hirosaki University Graduate School of Medicine
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- Yoshida Tatsuya
- Department of Vascular Biology, Hirosaki University Graduate School of Medicine
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- Shimada Taku
- Department of Vascular Biology, Hirosaki University Graduate School of Medicine
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- Hayakari Ryo
- Department of Vascular Biology, Hirosaki University Graduate School of Medicine
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- Kawaguchi Shogo
- Department of Gastroenterology and Hematology, Hirosaki University Graduate School of Medicine
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- Yoshida Hidemi
- Department of Vascular Biology, Hirosaki University Graduate School of Medicine
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- Kobayashi Tamotsu
- Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital
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- Hirabayashi Takeshi
- Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital
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- Mizukami Hiroki
- Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine
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- Kijima Hiroshi
- Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine
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- Hakamada Kenichi
- Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital
書誌事項
- タイトル別名
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- Retinoic Acid‐inducible Gene‐I and CXCL10 are Involved in Biliary Atresia
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Purpose: Retinoic acid-inducible gene-I(RIG-I)is a member of cytoplasmic viral sensors which plays an important role in inflammation of biliary epithelial cells(BECs). The aim of this study is to examine if RIG-I and C-X-C motif chemokine 10(CXCL10)are involved in the etiology of human biliary atresia(BA).<br> Methods: Immunohistochemical study was performed on surgically resected tissues obtained(June 1994 to March 2016) from 30 infants with BA and non-inflamed hepatic tissues from 7 infants with hepatoblastoma. A semiquantitative scoring system was designed to evaluate the staining with an antibodies to the RIG-I and CXCL10. The expression of RIG-I and CXCL10 in HuCCT1 cholangiocarcinoma cell line were studied by western blotting, ELISA and RT-PCR analyses.<br> Results: Intense immunoreactivity for RIG-I and CXCL10 was detected in BECs in tissues resected from BA patients. The expression of RIG-I and CXCL10 in the hilar tissue was significantly stronger than in the hepatic tissue. Transfection of HuCCT1 cells with poly(I:C), a synthetic analog of viral dsRNA, induced the expression of RIG-I, and knockdown of RIG-I inhibited the induction of CXCL10 in HuCCT1 cells transfected with poly(I:C).<br> Conclusion: These results suggest that RIG-I-CXCL10 cascade may be involved in the etiology of human BA.
収録刊行物
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- 弘前医学
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弘前医学 69 (1-4), 86-94, 2019-03-15
弘前大学大学院医学研究科・弘前医学会
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詳細情報 詳細情報について
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- CRID
- 1390001288141282048
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- NII論文ID
- 130007656021
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- NII書誌ID
- AN00211444
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- ISSN
- 24344656
- 04391721
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- HANDLE
- 10129/00006586
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- NDL書誌ID
- 029661069
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- CiNii Articles
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- 使用不可