High frequency plant regeneration from leaf culture of Neolamarckia cadamba

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  • Li Jingjian
    College of Forestry and Landscape Architecture, South China Agricultural University State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm
  • Zhang Deng
    College of Forestry and Landscape Architecture, South China Agricultural University
  • Ouyang Kunxi
    College of Forestry and Landscape Architecture, South China Agricultural University
  • Chen Xiaoyang
    College of Forestry and Landscape Architecture, South China Agricultural University State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm

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  • High frequency plant regeneration from leaf culture of <i>Neolamarckia cadamba</i>

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<p>Neolamarckia cadamba is a miracle tree species with considerable economic potential uses as a timber wood, woody forage and traditional medicine resource. The present study aimed to establish a highly efficient and robust protocol of plant regeneration for N. cadamba. Greenish callus was induced from very young leaf explants of sterile in vitro plantlets cultured on Murashige and Skoog’s (MS) medium supplemented with 3 mg l−1 thidiazuron (TDZ), 0.1 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.05 mg l−1 α-naphthaleneacetic acid (NAA). The callus could differentiate into nodular embryogenic structures or adventitious shoots, and these two regeneration pathways often occurred in the same callus clumps. The micro-shoots developed roots in MS supplemented with 0.05 mg l−1 NAA and 0.05 mg l−1 indole-3-butyric acid (IBA), while the nodular embryogenic structures germinated directly and developed into plantlets on induction medium contained with 0.5 mg l−1 (or 1 mg l−1) 6-benzyladenine (6-BA) and 0.05 mg l−1 NAA. The rooted plantlets could be successfully acclimatized to a greenhouse with more than 92.0% survival. This regeneration protocol can be used in large scale cultivation needs and may be useful for future genetic modifications of N. cadamba.</p>

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