Denture Adhesive Used as Gap Filler when Using a Cooling System Installed Microtome: Effects on Preparation of Thin Serial Sections

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  • Saio Masanao
    Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences
  • Hatori Mizuho
    Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences
  • Moriya Syunichi
    Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences
  • Ogawa Mana
    Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences
  • Hotoyama Hiroyu
    Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences
  • Fujimori Misa
    Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences
  • Nomi Yuri
    Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences
  • Kobayashi Sayaka
    Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences
  • Kimura Kiminori
    Department of Hepatology, Tokyo Metropolitan Cancer and Infectious Disease Center, Komagome Hospital

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  • パラフィンブロック冷却装置付きミクロトームと義歯安定剤のブロック裏面隙間充填による連続薄切への効果の検討
  • パラフィンブロック レイキャク ソウチ ツキ ミクロトーム ト ギシ アンテイザイ ノ ブロック リメン スキマ ジュウテン ニ ヨル レンゾク ウスギリ エ ノ コウカ ノ ケントウ

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Objectives: We evaluated the usefulness of a cooling system and the use of denture adhesive (DA) as a gap filler in preparing thin serial sections with a sliding microtome.<br>Methods: We prepared paraffin blocks alone (i.e., no patient sample) and initially examined how DA affected paraffin block cooling by the cooling apparatus. Then, we examined how thin serial sections could be prepared using the cooling system installed in the sliding microtome.<br>Results: With DA, differences in the block surface temperature between 0-5 min and between 0-20 min after cooling were significantly larger than that without DA, although the block temperature (20 min) was not significantly different with or without DA. Additionally, the cooling system installed in the microtome enabled the generation of 1μm-thick serial sections.<br>Conclusions: In daily pathology practice, we typically pre-cool blocks using an ice plate or a cooling equipment, but we do not cool the block during sectioning. This condition sometimes leads to difficulty in preparing serial sections. To solve this problem, a cooling system was installed in the microtome.

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