Differential Chromosome Banding of Three <i>Rauvolfia</i> Species

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<p>Three species of Rauvolfia L. viz. R. serpentina L. Benth. ex Kurz, R. vomitoria Afz. and R. tetraphylla L. were studied with differential banding for cytogenetic characterization. R. serpentina and R. vomitoria were found to possess 2n=22 chromosomes, whereas 2n=66 in R. tetraphylla. The three species have distinct chromomycin A3 (CMA)- and 4′, 6-diamidino-2-phenylindole (DAPI)-banding patterns in respect of the number, location, and relative size to complement. No satellite was observed in the three species after orcein and DAPI-banding. However, a pair of satellites was found in R. vomitoria and R. tetraphylla after CMA-staining. In addition, R. vomitoria and R. tetraphylla showed similarities in some karyomorphological features which suggested that these species might share some common genome.</p>

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