Study on Nanoparticle Sizing Using Fluorescent Polarization Method with DNA Fluorescent Probe
-
- Hayashi Terutake
- Kyushu University
-
- Ishizaki Yuki
- Osaka University
-
- Michihata Masaki
- Osaka University
-
- Takaya Yasuhiro
- Osaka University
-
- Tanaka Shin-ichi
- Kure National College of Technology
Bibliographic Information
- Other Title
-
- Study on Nanoparticle Sizing Using Fluorescent Polarization Method with DNA Fluorescen Probe
Search this article
Abstract
<p>Fluorescent polarization methods are used to detect complementary base pairing of DNA in biological fields. These methods work by measuring the rotational diffusion coefficient of Brownian motion of the fluorescent particles in solution. The rotational diffusion coefficient corresponds to the inverse third power of diameter according to the Debye-Stokes-Einstein equation for nanoparticles as hard spheres. We develop a novel method to measure the rotational diffusion coefficient using a fluorescent probe with a DNA spacer connected to a gold nanoparticle. We studied the physical characteristics of this probe to verify the feasibility of the proposed method. The rotational diffusion coefficients of gold nanoparticles with diameters ranging between 5–20 nm were measured using this developed system. In this manuscript we describe a novel fluorescent polarization method for nanoparticle sizing using a fluorescent DNA probe.</p>
Journal
-
- International Journal of Automation Technology
-
International Journal of Automation Technology 9 (5), 534-540, 2015-09-05
Fuji Technology Press Ltd.