A Method for Morphologic Evaluation of Erythrocytes in Acid-Base Balance Disorder

DOI Web Site
  • Soyama Tomonori
    Health Care Education and Research Center, Tenri Health Care University
  • Kuroda Kyota
    Department of Central Clinical Laboratory, Nara Prefecture General Medical Center

Bibliographic Information

Other Title
  • 酸塩基平衡異常における赤血球形態の観察方法
  • サンエンキ ヘイコウ イジョウ ニ オケル セッケッキュウ ケイタイ ノ カンサツ ホウホウ

Search this article

Abstract

<p>　A critical situation erythrocyte aggregation appearing during cardiopulmonary bypass procedure is said to be caused from echinocytosis in alkalemia. Ordinary smear investigation,however, is difficult to evaluate morphology of erythrocytes in acid-base abnormalities. In this report, we tried to see erythrocytes morphology in alkalemia using red cells suspension. Red cell suspensions with different acid-base balance were prepared and plated in culture plates. Using inverted microscope, erythrocytes were evaluated from the bottom of the plates. We can see normal erythrocytes in red cell suspension with pH 7.5 without influence of glass effect, an artificial morphologic abnormality usually observed using glass slides and cover slides. On the other hand, plentiful erythrocytes with pH 9.0 showed morphologic abnormalities so called echinocyto. These findings show an effective method for erythrocytes morphology in acid-base abnormalities. Also, echinocyto are appeared in alkalemia, which is candidate cause of erythrocyte aggregation occurring in cardiopulmonary bypass in surgery.</p>

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top