Super-Resolution Microscopy Based on the Confocal Microscope Optics

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  • Okada Yasushi
    理化学研究所・生命システム研究センター 東京大学大学院理学系研究科

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  • 共焦点顕微鏡の光学系を用いた超解像顕微鏡法
  • キョウ ショウテン ケンビキョウ ノ コウガクケイ オ モチイタ チョウカイゾウ ケンビキョウホウ

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Description

<p>For the observation of moving objects, the image acquisition time should be short enough to avoid motion blur. However, most super-resolution fluorescent microscope techniques achieved high spatial resolution at the expense of temporal resolution, which precluded their application to live cell imaging. Here, we discuss the principle of the super-resolution microscopy based on the confocal microscope optics, which enabled us to observe the dynamics of subcellular organelles in living cells at the temporal resolution of 10 ms (100 frames per sec) and the spatial resolution around 100 nm (twice better than the diffraction limit).</p>

Journal

  • KENBIKYO

    KENBIKYO 52 (2), 62-66, 2017-08-30

    The Japanese Society of Microscopy

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