The effects of Eushearilide, a novel macrolide antifungal antibiotic, on rat mitochondrial respiratory function
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- Kitagawa Akira
- Department of Nutrition, Shigakkan University
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- Hosoe Tomoo
- Faculty of Pharmaceutical Sciences, Hoshi University
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- Kawai Ken-ichi
- Faculty of Pharmaceutical Sciences, Hoshi University
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- Kitagawa Hiroyuki
- Department of Veterinary Medicine, Nihon University College of Bioresource Sciences
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- Kawai Kiyoshi
- Department of Nutrition, Shigakkan University
Bibliographic Information
- Other Title
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- 新規マクロライド系抗真菌抗生物質,Eushearilideのラット肝ミトコンドリアの呼吸機能に及ぼす影響
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Abstract
<p> Eushearilide, a newly isolated macrolide antibiotic isolated from Eupenicillium shearii, has been examined for its toxic effects on mitochondrial respiration and structure to gain insight into the molecular mechanism for the antifungal activity, by using freshly isolated rat liver mitochondria. Eushearilide inhibited the respiration oxidizing both L-glutamate and succinate. The inhibition was not reversed by N,N,N',N'-tetramethyl-1,4-phenylenediamine dihydrochloride, which generates an electron transport shunt over the inhibition sites of rotenone and antimycin A, and was not restored by ascorbate, an artificial substrate for the cytochrome c oxidase. The all of cytochromes a, b and c were kept reduced in the presence of eushearilide in the reducedminus oxidized difference spectrum of cytochromes. Eushearilide induced a large amplitude swelling in the mitochondria suspended in an isotonic KCl solution. This amplitude swelling was prevented by cyclosporine A, indicating the generation of the permeability pore in the inner membrane. These results suggested that eushearilide could impair mitochondrial respiratory function by inhibiting the electron transport at the site of complex IV (cytochrome c oxidase) in the respiratory chain and by inducing swelling.</p>
Journal
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- JSM Mycotoxins
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JSM Mycotoxins 69 (2), 65-69, 2019-07-31
Japanese Society of Mycotoxicology
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Details 詳細情報について
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- CRID
- 1390001288157630080
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- NII Article ID
- 130007695994
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- NII Book ID
- AA12721071
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- ISSN
- 18810128
- 02851466
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- NDL BIB ID
- 029958579
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed