Cryo-Electron Microscopy of Vitreous Sections
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- Iwasaki Kenji
- 大阪大学蛋白質研究所 CREST, JST
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- Miyazaki Naoyuki
- 大阪大学蛋白質研究所 CREST, JST
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- Ito Yoshiko
- CREST, JST ライカマイクロシスムズ(株)
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- Takagi Junichi
- 大阪大学蛋白質研究所
Bibliographic Information
- Other Title
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- CEMOVIS試料作製法
- CEMOVIS シリョウ サクセイホウ
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Description
<p>Cryo-electron microscopy of vitreous sections (CEMOVIS) enables us to observe hydrated biological materials that are cut into thin sections following physical fixation by high-pressure freezing. Thin sectioning conventionally embedded specimens causes certain artifacts, including a lack of molecules that are unfixed and deformation of molecules through chemical fixation, dehydration, and heavy metal staining. On the other hand, by avoiding the abovementioned processes, vitrification yields specimens that are closer to the native state. However, some artifacts caused by direct sectioning of vitrified ice have been verified since this technique first appeared. Here, we introduce the pros and cons of CEMOVIS, the methods for utilization of CEMOVIS, and the protocol and materials necessary to implement it.</p>
Journal
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- KENBIKYO
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KENBIKYO 47 (1), 44-50, 2012-03-30
The Japanese Society of Microscopy
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Details 詳細情報について
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- CRID
- 1390002184853186944
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- NII Article ID
- 10030552251
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- NII Book ID
- AA11917781
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- ISSN
- 24342386
- 13490958
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- NDL BIB ID
- 023682669
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed