A New Histochemical Approach to Localize Methylation Sites of DNA: HELMET

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  • Koji Takehiko
    長崎大学大学院医歯薬学総合研究科生命医科学講座組織細胞生物学分野

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  • HELMET法―DNAメチル化部位局在のための新しい組織化学
  • HELMETホウ : DNA メチルカ ブイキョクザイ ノ タメ ノ アタラシイ ソシキ カガク

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Abstract

<p>Epigenetic modification such as DNA methylation is implicated in the induction of cell differentiation and its maintenace in eukaryotes. In spite of potential importance of the analysis of methylation states of sequence specific DNA in indivisual cells, it was impossible. Here we presented a new histochemical method, HELMET, which permits us to analyze methylated levels of specific sites of DNA using an isoshizomeric set of restriction enzymes. When the method was applied to examine the methylation states of CCGG sequences in mouse testis, we effectively found differetiation stage-dependent changes in the ratio of the number of nonmethylated CCGG sites to that of methylated ones during spermatogenesis.</p>

Journal

  • KENBIKYO

    KENBIKYO 47 (1), 55-58, 2012-03-30

    The Japanese Society of Microscopy

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