Efficient transient gene expression system using buckwheat hypocotyl protoplasts for large-scale experiments
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- Sakamoto Shingo
- Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
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- Matsui Katsuhiro
- Institute of Crop Science, National Agriculture and Food Research Organization (NARO)
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- Oshima Yoshimi
- Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
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- Mitsuda Nobutaka
- Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
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Description
<p>Buckwheat (Fagopyrum esculentum) is cultivated worldwide and its flour is used in a variety of food products. Although functional analyses of genes in buckwheat are highly desired, reliable methods to do it have yet to be developed. In this study we established a simple and efficient transient gene expression system using buckwheat protoplasts isolated from young hypocotyls using 96-well plates as a high-throughput platform. The transformation efficiency was comparable with that of similar systems, such as Arabidopsis mesophyll protoplasts. Stable results were obtained in a typical example of the experiment to examine transcription factor activity. This system shows potential for the large-scale analysis of gene function using protoplast isolated from fewer and younger plants than the conventional system and may provide novel information for efficient buckwheat breeding.</p>
Journal
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- Breeding Science
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Breeding Science 70 (1), 128-134, 2020
Japanese Society of Breeding
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Keywords
Details 詳細情報について
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- CRID
- 1390002184882907904
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- NII Article ID
- 130007809717
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- NII Book ID
- AA11317194
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- ISSN
- 13473735
- 13447610
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- NDL BIB ID
- 030395511
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed