書誌事項
- タイトル別名
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- Endothelin-1 stimulates transcription of cyclin D1 and Skp2 through activation of Stat3 in cultured rat astrocytes
抄録
<p>Activation of Stat3, a member of the Stat family of transcription factors, plays a pivotal role in induction of reactive astrocytes and glial scar formation. Endothelin-1 (ET-1) increases in brain disorders and promotes astrocytic proliferation through ETB receptors. In this study, to clarify mechanisms underlying astrocytic proliferation, the effects of ET-1 on Stat3 were examined in rat cultured astrocytes. Treatment with ET-1 stimulated Ser727 phosphorylation of Stat3 in cultured astrocytes, although Tyr705 phosphorylation was not affected. ET-1 stimulated the binding of Stat3 protein to its consensus DNA fragments. ET-induced BrdU incorporation was reduced by Stat3 inhibitors and Stat3 siRNA. ET-1 increased the expression of cyclin D1 and Skp2 in cultured astrocytes. The effects of ET-1 on cyclin D1 and Skp2 expression were reduced by stattic, 5,15-DPP and Stat3 siRNA. ChIP-PCR analysis showed that ET-1 promoted the binding of Stat3 to 5'-flanking regions of rat cyclin D1 and Skp2 genes. These results suggest that cyclin D1 and Skp2 expression through Stat3-mediated mechanisms underlies ET-induced astrocytic proliferation.</p>
収録刊行物
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- 日本薬理学会年会要旨集
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日本薬理学会年会要旨集 92 (0), 2-O-01-, 2019
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390002184884130432
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- NII論文ID
- 130007812865
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- ISSN
- 24354953
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可