Application of High-Speed Countercurrent Chromatography to the Separation of Flavanonol, Phenylcoumaran and Flavonolignans in Silybum marianum
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- ZAIMA Kazumasa
- School of Pharmacy, Nihon University
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- KOGA Risako
- School of Pharmacy, Nihon University
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- MOTEGI Riku
- School of Pharmacy, Nihon University
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- SATO Hanae
- School of Pharmacy, Nihon University
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- KITANAKA Susumu
- School of Pharmacy, Nihon University
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- ITO Yoichiro
- Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, Division of Intramural Research, National Heart, Lung, and Blood Institute, National Institutes of Health
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- SHINOMIYA Kazufusa
- School of Pharmacy, Nihon University
書誌事項
- タイトル別名
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- Application of High-Speed Countercurrent Chromatography to the Separation of Flavanonol, Phenylcoumaran and Flavonolignans in <i>Silybum marianum</i>
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説明
<p>Separation of flavanonol, phenylcoumaran and flavonolignans in Silybum marianum was examined using high-speed countercurrent chromatography (HSCCC). In order to prepare analytical standards, a flavanonol of aromadendrin (87.4% purity, 7.8 mg), three phenylcoumarans of jatrointelignan D (84.5% purity, 13.2 mg), dehydrodiconiferyl alcohol (76.1% purity, 1.4 mg) and dihydrodehydrodiconiferyl alcohol (93.0% purity, 5.8 mg), and three flavonolignans of silybin (88.8% purity, 35.6 mg), silydianin (99.3% purity, 25.1 mg) and silychristin (96.9% purity, 12.6 mg) were separated from the seeds of S. marianum using common column chromatography and ODS-HPLC, and identified by 1H and 13C NMR spectra. Then, HSCCC with the hexane/ethyl acetate/methanol/water (3 : 7 : 4 : 6, v/v) system was applied to the separation of aromadendrin, jatrointelignan D, silydianin and silybin. In this separation, it was revealed that silybin and silydianin were successfully separated from each other. The present HSCCC system was directly applied to the ethyl acetate extract and resulted in the separation of silybin. The overall results suggested that HSCCC is useful for the separation of bioactive compounds in S. marianum.</p>
収録刊行物
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- CHROMATOGRAPHY
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CHROMATOGRAPHY 41 (2), 91-95, 2020-06-20
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詳細情報 詳細情報について
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- CRID
- 1390003825196321920
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- NII論文ID
- 130007870140
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- NII書誌ID
- AA1137755X
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- ISSN
- 13483315
- 13428284
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- NDL書誌ID
- 030540235
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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