Direct Determination of Protein Soil on Fabric by the Reduction of Bicinchoninic Acid

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  • ビシンコニン酸の還元反応による布帛上のたんぱく質汚れの直接定量

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Abstract

<p>  To determine colorless soil components such as proteins adhered to fabric products is difficult without professional and expensive equipment, skilled techniques and long analysis time. In this study, we have focused on the bicinchoninic acid method used in solution systems. The objective of this study is to examine the direct determination of protein soils on fabrics as the substrate. The surface reflectance was measured and evaluated directly for the color reaction on the fabric. As a result, a good linear relationship was obtained between the surface reflectance and the albumin concentration used as a model protein soil. In addition, no problems were found for this color reaction with coexistence of residual surfactants in normal cleaning or a fatty acid. Compared with conventional methods, it revealed that the present method can be performed under normal laboratory conditions, and has superiority in accuracy, simplicity, and economics.</p>

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