Isolation of Nitrogen-Fixing Bacteria from Water Yam (<i>Dioscorea alata</i> L.)

DOI Open Access

Description

<p>The results of our previous experiment showed that water yam (Dioscorea alata L.) was able to grow under low fertility soil conditions without nitrogen fertilization. Moreover, changing δ15N values suggested that nitrogen is absorbed from the air. The objective of this study, therefor, was to confirm and identify the nitrogen-fixing bacteria in water yam plants. The nitrogen fixation ability of isolated bacteria was measured using the acetylene reduction assay in semi-solid nitrogen free medium. Phylogenetic analysis of 16S rRNA gene sequences revealed nine genera in the stems and roots. These isolates were shown to be related to genus Bacillus, Cohnella, Rhizobium, Kosakonia, Azospirillum, Pantoea, Dyella, Enterobacter, and Ralstonia. This is the first result that NFB were isolated from water yam plant cultivated under low soil fertility condition at Miyako Island, Japan.</p>

Journal

Related Projects

See more

Keywords

Details 詳細情報について

  • CRID
    1390004222614672768
  • NII Article ID
    130007898141
  • DOI
    10.11248/jsta.63.198
  • ISSN
    18828469
    18828450
  • Text Lang
    en
  • Data Source
    • JaLC
    • CiNii Articles
    • KAKEN
  • Abstract License Flag
    Disallowed

Report a problem

Back to top