Establishment of reporter assay yeast strain for detecting ligands of <i>Daphnia magna</i> juvenile hormone receptor <i>via</i> upstream E-box and E-box-like elements of the <i>Vrille</i> gene

DOI
  • ITO-HARASHIMA Sayoko
    Department of Biological Science, Graduate School of Science, Osaka Prefecture University
  • OGAWA Masahiro
    Department of Biological Science, Graduate School of Science, Osaka Prefecture University Life Science Research Institute, Kumiai Chemical Industry Co. Ltd.
  • KYOYA Takahiro
    Life Science Research Institute, Kumiai Chemical Industry Co. Ltd.
  • TERADA Megumi
    Life Science Research Institute, Kumiai Chemical Industry Co. Ltd.
  • KAWANISHI Masanobu
    Department of Biological Science, Graduate School of Science, Osaka Prefecture University
  • YAGI Takashi
    Department of Biological Science, Graduate School of Science, Osaka Prefecture University

Bibliographic Information

Other Title
  • 性決定因子<i>Doublesex1</i>の発現を司る<i>Vrille</i>遺伝子上流のE-box/E-box様配列を介したオオミジンコ幼若ホルモン受容体リガンド検出酵母株の樹立

Abstract

<p>Juvenile hormones (JHs), which control growth and development of arthropods, induce male offspring production in daphnids by activating JH receptor methoprene-tolerant (Met). However, target genes of Met have been unidentified. In this study, we showed that JH activities were detectable in yeast expressing Daphnia magna Met via upstream E-box/E-box-like elements of Vrille (Vri) gene, which encodes transcriptional activator of sex-determining factor Doublesex1. We also showed that Vri was an early JH-responsive gene in vivo. These observations suggested that Vri is a target of Met in D. magna.</p>

Journal

Details 詳細情報について

  • CRID
    1390004222614763776
  • NII Article ID
    130007898237
  • DOI
    10.14869/toxpt.47.1.0_o-5
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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