Biochemical characterization of the jasmonic acid methyltransferase gene from wasabi (Eutrema japonicum)
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- Koeduka Takao
- Graduate School of Sciences and Technology for Innovation, Yamaguchi University
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- Suzuki Hideyuki
- Department of Research and Development, Kazusa DNA Research Institute
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- Taguchi Goro
- Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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- Matsui Kenji
- Graduate School of Sciences and Technology for Innovation, Yamaguchi University
書誌事項
- タイトル別名
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- Biochemical characterization of the jasmonic acid methyltransferase gene from wasabi (<i>Eutrema japonicum</i>)
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<p>Methyl jasmonate and jasmonic acid play important roles as signaling molecules in regulating plant development and stress-related responses. Previous studies have shown that jasmonic acid carboxyl methyltransferase (JMT), which belongs to the SABATH methyltransferase gene family, catalyzes the transfer of methyl groups from S-adenosyl-L-methionine to the carboxyl groups of jasmonic acid. In the present study, we used RNA-seq analysis to identify a putative JMT gene, EujJMT, in wasabi (Eutrema japonicum). The EujJMT proteins showed the highest similarity (89% identity) to JMT proteins of Brassica rapa. Functional characterization of a recombinant EujJMT protein expressed in Escherichia coli showed the highest level of activity with jasmonic acid among the different carboxylic acids tested. The apparent Km value of EujJMT using jasmonic acid as substrate was 62.6 µM, which is comparable to the values of known JMTs. Phylogenetic analysis suggested that EujJMT shares a common ancestor with the JMTs of Arabidopsis and Brassica species and that the strict substrate specificity toward jasmonic acid is conserved among Brassicaceae JMTs.</p>
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 37 (3), 389-392, 2020-09-25
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390004222622115968
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- NII論文ID
- 130007907504
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- NII書誌ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 030694383
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- CiNii Articles
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