-
- 末次 正幸
- 立教大学理学部生命理学科
書誌事項
- タイトル別名
-
- <i>In vitro</i> Reconstitution of Replication Cycle of the <i>Escherichia coli</i> Chromosome and its Impact on Synthetic Biology
- ダイチョウキン センショクタイ フクセイ サイクル ノ シケンカン ナイ サイコウセイ ト ゴウセイ セイブツガク
この論文をさがす
抄録
<p>In vitro reconstitution of cellular events is a promising strategy as a bottom-up approach to build an artificial cell in the field of synthetic biology. Also, the reconstituted system sometimes provides a useful biotechnology tool for various fields. We recently reconstituted a whole replication cycle of the Escherichia coli chromosome in vitro as a part of the bottom-up synthetic biology. This replication cycle reaction (RCR) propagates circular DNA exponentially and precisely by autonomous repetition of the cycle in an isothermal reaction. Even genome-sized DNA (1 Mbp) can be propagated as circular DNA in RCR. We also developed a DNA assembly tool. The combination of the DNA assembly and RCR amplification enables cell-free synthesis of artificial large circular DNA, providing a powerful tool for a top-down study of the synthetic biology, in which living cells are modified at a whole genome level.</p>
収録刊行物
-
- 生物物理
-
生物物理 60 (5), 284-287, 2020
一般社団法人 日本生物物理学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390004222629214336
-
- NII論文ID
- 130007919207
-
- NII書誌ID
- AN00129693
-
- ISSN
- 13474219
- 05824052
-
- NDL書誌ID
- 030695420
-
- 本文言語コード
- ja
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可