A Method to Purify a Sample Derivatized with 2,4-Dinitrophenylhydrazine (DNPH) Using Solid Phase Extraction

DOI

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Other Title
  • 2,4-ジニトロフェニルヒドラジン（DNPH）誘導体化を対象とした固相抽出による試料精製方法の開発

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Description

<p>2,4-Dinitrophenylhydrazine (DNPH) is one of the most common derivatizing reagent used for an analysis of aldehydes or ketones. Derivatives produced by this reaction are readily detected by UV/Vis photospectrometer or LC/MS. However, during the derivatization, an excess amount of DNPH is added to a sample and a large part of it remains unreacted. Injecting this residual DNPH into an analytical instrument may lead to some negative effects such as a damaged LC column, or a clogged sampling cone, probably resulting in an impaired detection sensitivity. It is therefore of importance to remove unreacted DNPH prior to an analysis.</p><p>The objective of this study is to develop a purification method for a DNPH-derivatized sample. Solid phase extraction (SPE) was focused on as a strategy to achieve this. The SPE purification method developed in this study removed 97.5％ of unreacted DNPH and recovered 103％ of a derivative. Additionally, quantum chemistry calculation was adopted to verify the experimental results. The calculation results were in good accordance with the experimental ones, i.e., the complexation between DNPH and a sorbent was more stable than that between a derivative and a sorbent.</p>

Journal

  • ENVIRONMENTAL SCIENCE

    ENVIRONMENTAL SCIENCE 33 (5), 70-78, 2020-09-30

    SOCIETY OF ENVIRONMENTAL SCIENCE, JAPAN

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Details 詳細情報について

  • CRID
    1390004222629240576
  • NII Article ID
    130007919238
  • DOI
    10.11353/sesj.33.70
  • ISSN
    18845029
    09150048
  • Text Lang
    en
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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