食中毒患者，ニワトリおよびウシ由来カンピロバクターのmultiplex PCR binary typing法による型別

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  • Multiplex PCR Binary Typing of <i>Campylobacter jejuni/coli</i> Isolates from Patient, Poultry and Bovine
  • ショクチュウドク カンジャ,ニワトリ オヨビ ウシ ユライ カンピロバクター ノ multiplex PCR binary typingホウ ニ ヨル カタベツ

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<p>The multiplex PCR binary typing (mP-BIT) is applied to know the relationship among 386 Campylobacter jejuni/coli isolates obtained from patient, bovine, and poultry. As the result of this, two major clusters were observed consist of patient and poultry isolates. One of these clusters contains 27 isolates of patients from 13 cases (11 of 13 cases are poultry related) and 12 poultry isolates. The isolates including this major cluster have 10 genes by mP-BIT. Another cluster contains 19 isolates of patients from 9 cases (7 of 9 cases are poultry related) and 19 poultry isolates. Though little bovine isolates form cluster with patient isolates, major cluster consists only bovine isolates each other. Consequently, human foodborne campylobacteriosis might be mainly related to poultry isolates, on the other hand, only a part of the bovine isolates are related to human foodborne campylobacteriosis. These 386 isolates are also classified by Penner serotyping. As the result of this, Penner O and L are major (both 11 of 159 strains) among patient isolates, Penner D is the major (24 of 91 strains) among bovine isolates, Penner O (11 of 76 strains) is the major among poultry isolates. Though, major isolates (243 of 386; 63%) are Penner serotyping untypeable.</p>

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