Three-dimensional analysis of ectomycorrhizas formed in <i>Pinus thunbergii</i> roots inoculated with <i>Rhizopogon roseolus</i>

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  • PRAMOJ NA AYUDHYA Sawithree
    1) The United Graduate School of Agricultural Sciences, 4-101 Koyamacho Minami, Tottori 680-8553, Japan
  • SUGIMURA Shin
    2) Faculty of Agriculture, Tottori University, 4-101 Koyamacho Minami, Tottori 680-8553, Japan
  • OZAKI Yuma
    1) The United Graduate School of Agricultural Sciences, 4-101 Koyamacho Minami, Tottori 680-8553, Japan
  • AIMI Tadanori
    2) Faculty of Agriculture, Tottori University, 4-101 Koyamacho Minami, Tottori 680-8553, Japan
  • SHIMOMURA Norihiro
    2) Faculty of Agriculture, Tottori University, 4-101 Koyamacho Minami, Tottori 680-8553, Japan

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Other Title
  • ショウロ菌を接種したクロマツ実生根における外生菌根の3次元解析
  • Three-dimensional analysis of ectomycorrhizas formed in Pinus thunbergii roots inoculated with Rhizopogon roseolus

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The detailed microstructure of the ectomycorrhizas formed in Pinus thunbergii roots inoculated with Rhizopogon roseolus was investigated using three-dimensional (3D) image analysis. When P. thunbergii seedlings were artificially inoculated with dichotomous R. roseolus, ectomycorrhizas appeared in seedling roots at 4 weeks after inoculation. The resulting ectomycorrhizas were used to prepare microscopic specimens for light microscopy observation. Using the serial sections, 3D images of Hartig net cells were constructed. The Hartig net cells were highly branched, and certain cells were common in mantle sheath and Hartig net. A constructed 3D image of mantle cells revealed that the outermost cells were cylindrical and the innermost cells were irregularly shaped. Cell volume measurements from the 3D image analysis revealed that the single cell volume of the most exterior and interior mantle sheaths, and the Hartig net measured 365, 452, and 1,516 μm3, respectively. This is the first case report on the 3D analysis of the cell volume of mantle and Hartig net cells in ectomycorrhizas.

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