Tracking the subcellular protein localization process by combining the time-gated method and photoconvertible fluorescence protein
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- Sakata Momoko
- Center for Bioscience Research and Education, Utsunomiya University United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
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- Kodama Yutaka
- Center for Bioscience Research and Education, Utsunomiya University United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
Bibliographic Information
- Other Title
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- タイムゲート法と光変換型蛍光タンパク質とを組み合わせた細胞内タンパク質局在過程の追跡
- タイムゲートホウ ト ヒカリ ヘンカンガタ ケイコウ タンパクシツ ト オ クミアワセタ サイボウ ナイ タンパクシツキョクザイカテイ ノ ツイセキ
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Description
<p>Chlorophyll within chloroplast emits high-intensity autofluorescence, which interferes with the observation of exogenous fluorescent substance such as fluorescent protein in the plant cell. We previously reported that clear fluorescence observation can be done by using the time-gated method that eliminates the autofluorescence. Recently, we succeeded in tracking the subcellular localization process of the blue-light photoreceptor phototropin (Phot) to the chloroplast periphery in the liverwort Marchantia polymorpha by a combination of the confocal laser microscopy for the time-gated method and a photoconvertible fluorescent protein. Here, as an example of the time-gated fluorescence imaging, we introduce a study about intracellular localization and movement of Phot in M. polymorpha.</p>
Journal
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- PLANT MORPHOLOGY
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PLANT MORPHOLOGY 32 (1), 27-30, 2020
The Japanese Society of Plant Morphology
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Details 詳細情報について
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- CRID
- 1390006050801441408
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- NII Article ID
- 130008006490
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- NII Book ID
- AA11315461
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- ISSN
- 18844154
- 09189726
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- NDL BIB ID
- 031740786
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed