Atmospheric Pressure Plasma Treatment with Nitrogen Induces Osteoblast Differentiation and Reduces iNOS and COX-2 Expressions

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  • Sato Ryoichi
    Nihon University Graduate School of Dentistry
  • Namura Yasuhiro
    Department of Orthodontics, Nihon University School of Dentistry Division of Clinical Research, Dental Research Center, Nihon University School of Dentistry
  • Tanabe Natsuko
    Department of Biochemistry, Nihon University School of Dentistry Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry
  • Sakai Mayu
    Nihon University Graduate School of Dentistry
  • Utsu Akihisa
    Nihon University Graduate School of Dentistry
  • Tomita Keiko
    Nihon University Graduate School of Dentistry
  • Suzuki Naoto
    Department of Biochemistry, Nihon University School of Dentistry Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry
  • Motoyoshi Mitsuru
    Department of Orthodontics, Nihon University School of Dentistry Division of Clinical Research, Dental Research Center, Nihon University School of Dentistry

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<p>In recent years, atmospheric pressure plasma jet (APPJ) has been widely developed for various medical applications, such as medical equipment sterilization, gene transfection, cell proliferation, and wound healing. In particular, non-thermal APPJ enables direct treatment of the biological system without any thermal-associated damage. The effect of cells on APPJ depends upon the gas species used in the treatment. However, the mechanisms underlying osteoblast differentiation mediated by APPJ with nitrogen are yet to be studied. This study investigated the effects of nitrogen-APPJ on osteoblast differentiation by assessing the transcription factors, extracellular matrix proteins (ECMPs), alkaline phosphatase (ALP) activity, and the mRNA and protein expressions of ALP, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), in an osteoblast mouse cell line. We found that nitrogen-APPJ induced osteoblast differentiation-related transcription factors (runt-related transcription factor 2 [Runx2] and osterix), osteocalcin (OCN), and ALP activity, as well as reduced the mRNA and protein expressions of iNOS and COX-2. Thus, we concluded that APPJ affects differentiation of the osteoblast cells.</p>

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