Fluorescent Staining and FISH of rDNA of Pitaya (<i>Hylocereus</i> spp.) Chromosomes
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- YAMAMOTO Masashi
- Faculty of Agriculture, Kagoshima University
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- SHIMAJIRI Yohei
- Nago Branch, Okinawa Prefectural Agricultural Research Center
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- NASHIMA Kenji
- College of Bioresource Sciences, Nihon University
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- YAMAMOTO Toshiya
- Institute of Fruit Tree and Tea Science, NARO
Abstract
<p>Chromosome analysis of pitaya (Hylocereus spp.), a tropical fruit crop of the vine cactus, was conducted. All experiments were carried out using root tips derived from cutting plants as materials. Good chromosome samples all chromosomes relatively extended and well-spread without cytoplasm were prepared by the enzymatic maceration and air-drying method (EMA). The chromosome numbers of H. undatus, H. costaricensis, and H. megalanthus were 2n = 22, 22, and 44, respectively. In H. undatus and H. costaricensis, CMA-positive (+) bands were observed in telomeric positions of two chromosomes. DAPI-negative bands (–) corresponded to CMA+ bands. The CMA+/DAPI- bands were observed at the telomeric position of four chromosomes in H. megalanthus. When fluorescence in situ hybridization of rDNA was performed, the 18S-5.8S-25S rDNA sites were detected in telomeric positions of two chromosomes of H. undatus and H. costaricensis and four chromosomes of H. megalanthus. The 18S-5.8S-25S rDNA sites corresponded to the CMA+/DAPI- bands.</p>
Journal
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- Tropical Agriculture and Development
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Tropical Agriculture and Development 64 (4), 212-217, 2020
Japanese Society for Tropical Agriculture
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Details 詳細情報について
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- CRID
- 1390006308199386880
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- NII Article ID
- 130008028952
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- ISSN
- 18828469
- 18828450
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- Text Lang
- en
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed