Comparison of antimicrobial susceptibility tests of positive blood cultures performed by a rapid method using early-stage microcolonies and the standard method using a single colony

DOI Web Site
  • OI Yuka
    Department of Clinical Laboratory, Osaka General Medical Center
  • NISHIMOTO Erina
    Department of Clinical Laboratory, Osaka General Medical Center
  • WADA Honoka
    Department of Clinical Laboratory, Osaka General Medical Center

Bibliographic Information

Other Title
  • 血液培養陽性検体における分離培養初期の微小コロニー群を用いた測定と通常測定での薬剤感受性検査の比較検討
  • ケツエキ バイヨウ ヨウセイケンタイ ニ オケル ブンリ バイヨウ ショキ ノ ビショウ コロニーグン オ モチイタ ソクテイ ト ツウジョウ ソクテイ デ ノ ヤクザイ カンジュセイ ケンサ ノ ヒカク ケントウ

Search this article

Abstract

<p>Antimicrobial susceptibility tests of positive blood cultures are required for the timely treatment of patients with appropriate antibacterial drugs. Antimicrobial susceptibility tests using a single colony typically take three days to complete. Thus, we compared the antimicrobial susceptibility test results of a rapid method using microcolonies obtained after 2 to 4 hours of subculture with those of the standard method using a single colony. The target species were Escherichia coli and Klebsiella pneumoniae. The concordance rate was calculated for E. coli (225 samples) and K. pneumoniae (79 samples). The MIC 1 agreements of the rapid method and standard method were 97.3–100.0% for E. coli and 96.2–100.0% for K. pneumoniae, the category complete agreements were 81.8–100.0% for E. coli and 78.5–100.0% for K. pneumoniae, the major error rates were 0–0.4% for E. coli and 0–2.5% for K. pneumoniae, and the very major error rates were 0–0.4% for E. coli and 0–1.3% for K. pneumoniae. Thus, these findings suggest that the results of the antimicrobial susceptibility test performed using microcolonies formed in the early stage of subculture are reliable and can be reported quickly.</p>

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top