Spatiogenetic characterization of <i>S</i> receptor kinase (<i>SRK</i>) alleles in naturalized populations of <i>Raphanus sativus</i> L. var. <i>raphanistroides</i> on Yakushima island
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- Fukushima Kazuki
- Graduate School of Life Sciences, Tohoku University
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- Kanomata Toko
- Miyagi Prefecture Sendaidaiichi High School Exploring-Germination-and-Growth Program for Young Scientists, Tohoku University
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- Kon Aoi
- Graduate School of Life Sciences, Tohoku University
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- Masuko-Suzuki Hiromi
- Graduate School of Life Sciences, Tohoku University
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- Ito Kana
- Graduate School of Life Sciences, Tohoku University
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- Ogata Sadayoshi
- Graduate School of Life Sciences, Tohoku University
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- Takada Yoshinobu
- Graduate School of Life Sciences, Tohoku University
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- Komatsubara Yukihiro
- Miyagi Prefecture Sendaidaiichi High School
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- Nakamura Tsuyoshi
- Yakushima Environmental and Cultural Learning Center
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- Watanabe Takumi
- Yakushima Environmental and Cultural Learning Center
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- Koizumi Saori
- Yakushima Environmental and Cultural Learning Center
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- Sanuki Hitoshi
- Yakushima Environmental and Cultural Learning Center
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- Park Jong-In
- Department of Horticulture, Sunchon National University
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- Niikura Satoshi
- Tohoku Seed Co. Ltd.
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- Suwabe Keita
- Graduate School of Bioresources, Mie University
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- Fujii Sota
- Graduate School of Agriculture and Life Sciences, The University of Tokyo
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- Murase Kohji
- Graduate School of Agriculture and Life Sciences, The University of Tokyo PRESTO, Japan Science and Technology Agency (JST)
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- Takayama Seiji
- Graduate School of Agriculture and Life Sciences, The University of Tokyo
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- Suzuki Go
- Division of Natural Science, Osaka Kyoiku University
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- Watanabe Masao
- Graduate School of Life Sciences, Tohoku University Exploring-Germination-and-Growth Program for Young Scientists, Tohoku University
抄録
<p>In various coastal areas of Japan, naturalized radish populations are observed. Radish is a cruciferous plant and exhibits self-incompatibility, involving a system controlled by a single locus with multiple S alleles. Although the S allele diversity of radish cultivars and wild radishes has been characterized, the S allele distribution in naturalized populations has not yet been analyzed in relation to the positions of the plants in situ. Here, we show the S allele distribution in naturalized radish populations of Yakushima, a small island in the East China Sea, with positions of the plants. Radish plants were sampled in coastal areas in Yakushima, and their S alleles were detected and characterized. Most of the S alleles had been previously identified in radish cultivars. However, four novel S alleles, which may be unique to Yakushima, were also found. Moreover, seeds in siliques from plants growing in the study areas were sampled, and S allele determination in DNA extracted from these seeds suggested that the plants had exchanged their pollen among their close neighbors. There was also a problem in that the PCR amplification of some SRK alleles was difficult because of their sequence diversity in the naturalized populations, as occurs in cultivars. Our results suggest that the exchange of S alleles between cultivars and naturalized populations occurs and that S alleles in naturalized populations are highly diverse. The methodology established in our study should be applicable to other self-incompatible species to dissect the diversity of S allele distribution in naturalized populations.</p>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems advpub (0), 129-139, 2021
日本遺伝学会