A Study on RNA Detection and Infectivity of SARS-CoV-2 in Wastewater in Tokyo

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  • 東京都における下水中の新型コロナウイルスRNAの検出及び感染性に関する調査
  • トウキョウト ニ オケル カスイチュウ ノ シンガタ コロナウイルス RNA ノ ケンシュツ オヨビ カンセンセイ ニ カンスル チョウサ

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Abstract

<p>　There are few studies on the infectivity of SARS-CoV-2 in wastewater, although various studies reported the detection of its RNA from wastewater worldwide. From late June to late August 2020, Tokyo Metropolitan Government collected raw and treated wastewater from two wastewater treatment plants, whose catchments were assumed to have the large number of COVID-19 infection cases during the second wave of the pandemic. The samples were analyzed by RT-qPCR assay to detect SARS-CoV-2 RNA and cell culture-based assay to evaluate its infectivity. As a result of RT-qPCR assay, viral RNA was detected from some of the raw wastewater samples, but all of those concentrations were significantly low and less than the quantification limit. Additionally, cell culture-based assay resulted in no cytopathic effect peculiar to SARS-CoV-2, which suggests that its infectivity decayed. The comparison of the results of SARS-CoV-2 RNA with the number of new infection cases in each catchment showed that viral RNA was generally detected in raw wastewater when there were over four new cases per population of 100,000.</p>

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