Development of an <i>in vitro</i> culture system using decellularized tissue gels

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  • Ono Takuya
    Division of Rare Cancer Research, National Cancer Research Institute
  • Noguchi Rei
    Division of Rare Cancer Research, National Cancer Research Institute
  • Yoshimatsu Yuki
    Division of Rare Cancer Research, National Cancer Research Institute
  • Sin Yooksil
    Division of Rare Cancer Research, National Cancer Research Institute
  • Kojima Nobuhiko
    Department of Life and Environmental System Science, Yokohama City University
  • Kondo Tadashi
    Division of Rare Cancer Research, National Cancer Research Institute

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  • 脱細胞化ゲルによる新規培養系の確立

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Abstract

<p>The characteristics of tumor cells are greatly influenced by the tumor microenvironment, which contains multiple natural extracellular matrices (ECMs). However, only single or specific ECMs are presently used to assess the behavioral characteristics of tumor cells in vitro. Recently, as an alternative to conventional in vitro tumor models, to address the complex challenges in tumor in vitro assays, “decellularization” technology has recently emerged as a new platform for mimicking the tumor microenvironment. Especially, the use of decellularized tissue gels (DTGs) has gained attention as they provide multiple ECM components of the microenvironment of tumor cells. However, as tissues contain unique components of ECMs, it is essential to reproduce the tissue-specific ECM components when evaluating tumor functions in vitro. We hypothesized that DTGs affect the in vitro tumor cell behavior and that the protein composition of DTGs varies between tissues. To test this hypothesis, we created lung and liver DTGs via freeze-thawing and investigated the effects of DTGs on tumor cell behavior such as cell proliferation. We found that DTGs regulated the proliferation of tumor cells when the cells were cultured on DTGs-coated plates. The proteins present in the DTGs were separated via SDS-PAGE, and multiple bands were observed via silver staining. The band pattern varied depending on the tissue from which the DTGs were derived. It was found that certain DTGs modulated the behavioral characteristics of tumor cells, which may have potential applications in cancer studies and the development of therapies. Hence, DTGs are worth investigating as useful tools for tumor cell culture and in vitro assays.</p>

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