Low density cell culture enhances the formation of tight junction and hepatic function in HepG2 cells

DOI
  • TANAKA Rieko
    National Center for Child Health and Development
  • AIZAWA Kazuko
    National Center for Child Health and Development
  • ISO Manami
    National Center for Child Health and Development
  • SHIMIZU Kie
    National Center for Child Health and Development Graduate School of Science and Engineering, Saitama University
  • NAKAMURA Kazuaki
    National Center for Child Health and Development

Bibliographic Information

Other Title
  • 低密度播種培養によるHepG2の高機能化とそのメカニズム

Description

<p>HepG2, a liver cancer-derived cell line, is widely used as a human hepatocytes model. In this study, we had found that low-density cell culture induces hepatic function, so we examined the mechanism. Low-density culture increased CYPs gene expression and CYP3A4 activity in HepG2. Tight junction formation was also enhanced in low-density cultured cells, and occludin and tricellulin protein expression levels were also increased. These results suggest that low-density culture induces hepatic function via the promoting of tight junction formation. </p>

Journal

Details 詳細情報について

  • CRID
    1390007536269455360
  • NII Article ID
    130008073692
  • DOI
    10.14869/toxpt.48.1.0_p-149e
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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