Using qPCR method to find the number of transgenes inserted in the <i>Bombyx mori</i> genome
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- Kojima Katsura
- 国立研究開発法人 農業・食品産業技術総合研究機構 新素材開発グループ
Bibliographic Information
- Other Title
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- リアルタイムPCR法による遺伝子組換えカイコの挿入遺伝子数検証法について
- リアルタイム PCRホウ ニ ヨル イデンシ クミカエ カイコ ノ ソウニュウ イデンシスウ ケンショウホウ ニ ツイテ
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Description
<p> This report describes a method to ascertain the copy-number of transgenes of transgenic silkworm. For this study, probe-based quantitative polymerase chain reaction (probe qPCR) assay using comparative Ct method (ΔΔCt method) was used to detect the piggyBac sequence introduced into the silkworm genome. Using hsp90 gene as a reference, 0–8 copies of the piggyBac gene inserted into the silkworm genome were detected quantitatively.</p>
Journal
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- The Journal of Silk Science and Technology of Japan
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The Journal of Silk Science and Technology of Japan 29 (0), 59-65, 2021
The Japanese Society of Silk Science and Technology
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Keywords
Details 詳細情報について
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- CRID
- 1390007624812997120
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- NII Article ID
- 130008076720
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- NII Book ID
- AA11828182
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- ISSN
- 18811698
- 18808204
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- NDL BIB ID
- 031670470
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed