Chromosome behavior with 45S rDNA loci at meiosis of <i>Veronica persica</i>

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  • Fujimori Rika
    Department of Biological Sciences, Faculty of Science, Kanagawa University
  • Nemoto Tomomi
    Department of Biological Sciences, Faculty of Science, Kanagawa University
  • Inoue Yuri
    Department of Biological Sciences, Faculty of Science, Kanagawa University
  • Shimizu Saki
    Department of Biological Sciences, Faculty of Science, Kanagawa University
  • Lee Kyong Ye
    Department of Biological Sciences, Faculty of Science, Kanagawa University
  • Kumagai Kohei
    Department of Biological Sciences, Faculty of Science, Kanagawa University
  • Akinaga Kaoru
    Department of Biological Sciences, Faculty of Science, Kanagawa University
  • Toyoizumi Ryuji
    Department of Biological Sciences, Faculty of Science, Kanagawa University
  • Azumi Yoshitaka
    Department of Biological Sciences, Faculty of Science, Kanagawa University

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  • Chromosome behavior with 45S rDNA loci at meiosis of Veronica persica

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<p>Meiosis is essential for gamete production, and involves two rounds of successive cell divisions, meiosis I and II. During prophase of meiosis I, chromosomes present meiosis-specific behavior, such as homologous chromosomes (HCs) pairing and synapsis. Here, we present the results found in the observation of meiosis in Veronica persica (2n = 28). The chromosome behavior of pollen mother cells (PMCs) at meiosis was evaluated via 4′,6-diamidino-2-phenylindole (DAPI)-staining and fluorescent in situ hybridization (FISH) using as the probes 5.8S rRNA and 25S rRNA-encoding sequences to detect 45S rDNA locus. DAPI staining confirmed the chromosome number and revealed conformational changes. The space occupied by the chromosomes within the PMCs seemed to shrink during late leptotene, which may assist HC pairing. 5.8S rDNA FISH signals were detected on seven bivalents, indicating that V. persica possesses seven 45S rDNA loci within its genome. Among the seven loci, some of them were associated with the nucleoli, but others were not. Taken together, this research has provided us new information concerning the overall and locus-specific dynamics of meiotic chromosomes in V. persica PMCs.</p>

Journal

  • Chromosome Science

    Chromosome Science 24 (1-2), 13-19, 2021

    THE SOCIETY OF CHROMOSOME RESEARCH

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