Forefront of fluorescence and bioluminescence imaging

DOI
  • NAGAI Takeharu
    The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Ibaraki, Japan

Bibliographic Information

Other Title
  • 蛍光・生物発光イメージングの最前線

Description

<p>Development of fluorescence imaging technology using fluorescent proteins has made it possible to visualize physiological functions in live sample. Nanoscopic technologies such as super-resolution imaging have also significantly progressed in recent years. However, since the irradiation of excitation light is inevitable in the fluorescence observation, problems such as phototoxicity and autofluorescence are unavoidable. On the other hand, bioluminescence imaging using luciferase is attracting attention. Bioluminescence, unlike fluorescence, does not require external light illumination, so auto-fluorescence and phototoxicity cannot cause. This advantage of bioluminescence over fluorescence has been recognized for some time, but because the emission signal is weak, it requires long exposure times of tens of minutes and has not been used for live imaging. Recently, however, bright bioluminescent proteins such as NanoLuc, Nano-lantern, and Akaluc have been developed, and live imaging by the bioluminescence has begun to be realized. In addition, some bioluminescent systems have been able to biosynthesize luminescent substrates, producing fully spontaneous animals and plants. In this lecture, I will introduce recent progress on technology development related to fluorescence and bioluminescence imaging and discuss future prospects.</p>

Journal

Details 詳細情報について

  • CRID
    1390008290063819392
  • NII Article ID
    130008105056
  • DOI
    10.11239/jsmbe.annual59.123
  • ISSN
    18814379
    1347443X
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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