In vitro reconstruction of ligament-like tissue using decellularized membrane

DOI
  • Kimura Tsuyoshi
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan
  • Suzuki Mika
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan
  • Hashimoto Yoshihide
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan
  • Takahashi Hironobu
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan
  • Shimizu Tatsuya
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan
  • Nakamura Naoko
    Department of Bioscience and Engineering, College of Systems Engineering and Science Shibaura Institute of Technology, Saitama, Japan
  • Kishida Akio
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan

Bibliographic Information

Other Title
  • 靭帯再生を目指した脱細胞化膜状組織によるin vitro組織再構築

Abstract

<p>Recently, a decellularized tissue, which is extracellular matrix assembly, have been focused as one of candidate biomaterials. Recently, decellularized tendon and ligament have been tried as alternative tendon and ligament for tissue regeneration. However, it is difficult to recellularize inside of them. So, in this study, we proposed a method that decellularized membrane was recellularized and fabricated for in vitro reconstruction of ligament-like tissue having living cells. The decellularized pericardiums were prepared by two decellularized methods. Cells such as fibroblast, myoblast, mesenchymal stem cell, were adhered and cultivated on the decellularized pericardiums. The ligament-like tissue was reconstructed by rolling of the recellularized pericardiums with keeping the cellular adhesion in the inside. These results suggest that the ligament-like tissue could be reconstructed by using the recellularized pericardium.</p>

Journal

Details 詳細情報について

  • CRID
    1390008290063886592
  • NII Article ID
    130008105384
  • DOI
    10.11239/jsmbe.annual59.469
  • ISSN
    18814379
    1347443X
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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