Detection of the magnetic fields of iPS cell-derived cardiomyocytes using simulated waveforms
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- Yamaguchi Takeshi
- Department of Anatomy and Neurobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan Applied Electronics Laboratory, Kanazawa Institute of Technology, Ishikawa, Japan
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- Adachi Yoshiaki
- Applied Electronics Laboratory, Kanazawa Institute of Technology, Ishikawa, Japan
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- Tanida Takashi
- Department of Anatomy and Neurobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan
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- Oka Yoshinobu
- Department of Pediatric Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan
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- Yoshida Takashi
- Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan
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- Takahashi Kenji
- Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan
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- Tanaka Masaki
- Department of Anatomy and Neurobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan
Bibliographic Information
- Other Title
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- シミュレーション波形を用いたiPS細胞由来心筋細胞の自発磁場検出
Abstract
<p>Currently, iPS cell-derived cardiomyocytes are important as cell materials, and a high-speed, automated quality evaluation method is required for their practical application. Focusing on the fact that magnetic field measurement is non-invasive, non-contact, and non-destructive, we investigated a method for evaluating cells by measuring the magnetic field with SQUID magnetometer. In this study, we developed an effective method for detecting small biological signals of cultured cells from measurement data. First, mathematical models that reproduce the action potentials of mouse iPS cell-derived cardiomyocytes were developed. Next, we simulated the magnetic field generated from the cultured cell population. Furthermore, deep learning was performed using the simulated magnetic field waveforms, and measurement data from cardiomyocytes were analyzed by the trained network. As a result, we succeeded in detecting magnetic signals that periodically correspond to the beats observed with an optical microscope and the field potentials, thus demonstrating the effectiveness of this method.</p>
Journal
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- Transactions of Japanese Society for Medical and Biological Engineering
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Transactions of Japanese Society for Medical and Biological Engineering Annual59 (Abstract), 509-509, 2021
Japanese Society for Medical and Biological Engineering
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Details 詳細情報について
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- CRID
- 1390008290064465792
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- NII Article ID
- 130008105514
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- ISSN
- 18814379
- 1347443X
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed