Damage measurement on T-cells and analysis of parameter dependency under ultrasound exposure
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- Kajita Naoya
- Graduate School of Bio-Applications and Systems Engineering. Tokyo University of Agriculture and Technology. Tokyo.Japan
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- Saito Tatsuya
- Graduate School of Bio-Applications and Systems Engineering. Tokyo University of Agriculture and Technology. Tokyo.Japan
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- Ito Yoshiki
- Graduate School of Bio-Applications and Systems Engineering. Tokyo University of Agriculture and Technology. Tokyo.Japan
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- Chikaarashi takumi
- Graduate School of Bio-Applications and Systems Engineering. Tokyo University of Agriculture and Technology. Tokyo.Japan
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- Omata Daiki
- Faculty of Pharma-Science. Teikyo University. Tokyo. Japan
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- Suzuki Ryo
- Faculty of Pharma-Science. Teikyo University. Tokyo. Japan
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- Masuda Kohji
- Graduate School of Bio-Applications and Systems Engineering. Tokyo University of Agriculture and Technology. Tokyo.Japan
Bibliographic Information
- Other Title
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- 超音波照射条件に対するT細胞の傷害計測とパラメータ依存性の解析
Abstract
<p>Because ultrasound irradiation causes damage on cells, we analyzed the damage on cells under ultrasound exposure by varying its parameters. We examined the suspension of CD8-OVA1.3 cells, which are T-cells of mice, in a well plate on the surface of the degassed water at a water temperature of 37 oC. From the bottom of a water tank, ultrasound irradiation was performed by targeting a suspension of cells. After irradiation, fluorescent reagents were used to calculate the cell viability.As a result, the survival rate was the lowest at a frequency of 3 MHz, when a continuous wave (100% of duty ratio) was applied with a sound pressure of 400 kPa-pp. On the other hand, when only the duty ratio is less than 20% without changing other parameters, the survival rate raised 100%. From the results, cell damage complexly related to various ultrasound parameters.</p>
Journal
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- Transactions of Japanese Society for Medical and Biological Engineering
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Transactions of Japanese Society for Medical and Biological Engineering Annual59 (Proc), 759-760, 2021
Japanese Society for Medical and Biological Engineering
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Details 詳細情報について
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- CRID
- 1390008290064491392
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- NII Article ID
- 130008105581
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- ISSN
- 18814379
- 1347443X
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed