Damage measurement on T-cells and analysis of parameter dependency under ultrasound exposure

DOI
  • Kajita Naoya
    Graduate School of Bio-Applications and Systems Engineering. Tokyo University of Agriculture and Technology. Tokyo.Japan
  • Saito Tatsuya
    Graduate School of Bio-Applications and Systems Engineering. Tokyo University of Agriculture and Technology. Tokyo.Japan
  • Ito Yoshiki
    Graduate School of Bio-Applications and Systems Engineering. Tokyo University of Agriculture and Technology. Tokyo.Japan
  • Chikaarashi takumi
    Graduate School of Bio-Applications and Systems Engineering. Tokyo University of Agriculture and Technology. Tokyo.Japan
  • Omata Daiki
    Faculty of Pharma-Science. Teikyo University. Tokyo. Japan
  • Suzuki Ryo
    Faculty of Pharma-Science. Teikyo University. Tokyo. Japan
  • Masuda Kohji
    Graduate School of Bio-Applications and Systems Engineering. Tokyo University of Agriculture and Technology. Tokyo.Japan

Bibliographic Information

Other Title
  • 超音波照射条件に対するT細胞の傷害計測とパラメータ依存性の解析

Abstract

<p>Because ultrasound irradiation causes damage on cells, we analyzed the damage on cells under ultrasound exposure by varying its parameters. We examined the suspension of CD8-OVA1.3 cells, which are T-cells of mice, in a well plate on the surface of the degassed water at a water temperature of 37 oC. From the bottom of a water tank, ultrasound irradiation was performed by targeting a suspension of cells. After irradiation, fluorescent reagents were used to calculate the cell viability.As a result, the survival rate was the lowest at a frequency of 3 MHz, when a continuous wave (100% of duty ratio) was applied with a sound pressure of 400 kPa-pp. On the other hand, when only the duty ratio is less than 20% without changing other parameters, the survival rate raised 100%. From the results, cell damage complexly related to various ultrasound parameters.</p>

Journal

Details 詳細情報について

  • CRID
    1390008290064491392
  • NII Article ID
    130008105581
  • DOI
    10.11239/jsmbe.annual59.759
  • ISSN
    18814379
    1347443X
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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