Study of osteocyte differentiation triggered by 3D spheroid culture

DOI
  • Kim Jeonghyun
    Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan
  • Adachi Taiji
    Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan

Bibliographic Information

Other Title
  • ３次元スフェロイド培養による骨細胞分化誘導の研究

Abstract

<p>Osteocyte is a commander to modulate the bone remodeling. While studies in osteocyte differentiation are mainly performed in a conventional 2D condition, we fabricated 3D spheroids reconstructed by mouse pre-osteoblast cells in an ultra-low attachment plate. As a result, in the absence of chemical osteogenesis differentiation supplements, the osteocyte gene expressions in the spheroid were greatly up-regulated within 2-day, compared to the 2D monolayer. Similarly, spheroids reconstructed by pre-chondrocyte cells also exhibited the osteocyte-likeness. In addition, we introduced a 3D-to-2D model by transferring the 3D spheroids to a normal cell-adhesive dish. Eventually, it became 2D-like structure after dissociation. In the 3D-to-2D model, the up-regulated osteocyte gene expressions were recovered to the level of monolayer. The results indicated that the cell condensed condition for osteocyte precursor cells in the 3D spheroid culture triggered osteocyte differentiation. Those spheroids are expected to become a new in vitro model for osteocyte studies.</p>

Journal

Details 詳細情報について

  • CRID
    1390008290064710272
  • NII Article ID
    130008105366
  • DOI
    10.11239/jsmbe.annual59.467
  • ISSN
    18814379
    1347443X
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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