Visualization of abscisic acid and 12-oxo-phytodienoic acid in immature <i>Phaseolus vulgaris</i> L. seeds using mass spectrometry imaging

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  • Enomoto Hirofumi
    Department of Biosciences, Faculty of Science and Engineering, Teikyo University Advanced Instrumental Analysis Center, Teikyo University

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  • 質量分析イメージング法を用いたインゲンマメ未熟種子中のアブシシン酸および12-オキソ-フィトジエン酸の可視化
  • シツリョウ ブンセキ イメージングホウ オ モチイタ インゲンマメ ミジュク シュシ チュウ ノ アブシシンサン オヨビ 12-オキソ-フィトジエンサン ノ カシカ

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<p>Some studies suggested that abscisic acid （ABA）, and the jasmonic acid related-compound, 12-oxo-phytodienoic acid （OPDA）, play crucial roles in seed development, dormancy, and germination.　However, a lack of suitable technique for visualizing ABA and OPDA has restricted the investigation of their biological mechanisms in the immature seeds.　Mass spectrometry imaging （MSI） is a powerful tool for visualization of metabolites in biological tissue.　Here, I discuss the MSI workflow using matrix-assisted laser desorption/ionization （MALDI） and desorption electrospray ionization （DESI）.　Next, I introduce our recent studies on the visualization of ABA and OPDA in immature Phaseolus vulgaris L. seeds by DESI-MSI and derivatization with Girard’s reagent T in MALDI-MSI analyses.　I end with a discussion on the future prospects of the application of MSI for the plant hormones analysis.</p>

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