Screening for High-Growth Mutants in Sporophytes of <i>Undaria pinnatifida</i> Using Heavy-Ion Beam Irradiation
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- Sato Yoichi
- Bio-Resources Business Development Division, Riken Food Co., Ltd. Nishina Center for Accelerator-Based Science, RIKEN
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- Hirano Tomonari
- Nishina Center for Accelerator-Based Science, RIKEN Faculty of Agriculture, University of Miyazaki
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- Hayashi Yoriko
- Nishina Center for Accelerator-Based Science, RIKEN
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- Fukunishi Nobuhisa
- Nishina Center for Accelerator-Based Science, RIKEN
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- Abe Tomoko
- Nishina Center for Accelerator-Based Science, RIKEN
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- Kawano Shigeyuki
- Future Center Initiative, The University of Tokyo
抄録
<p>In Undaria pinnatifida, an effective method for mutant screening in sporophytes has not been established. The present study developed a novel mutant screening method for Undaria sporophyte by combining gametophyte mutagenesis with heavy-ion beam and land-based tank culture system. When we irradiated gametophytes and sporophytes with carbon- and argon-ion beams, survival rates of the female gametophytes and the sporophytes decreased with increasing dose. However, those of the male gametophyte did not decrease after both of the irradiations. Mutant screening during the sporophyte development was performed by using a land-based tank culture system. High-growth plants were selected in the first mutant (M1) population derived from the irradiated materials. We successfully obtained mutant candidates with higher growth than the wild type in the M2 generation obtained from brother-sister inbreeding of selected M1 plants. Four high-growth mutant candidate lines were selected from M2 populations of 48 lines. The mutant candidates were derived from 3 lines of the gametophyte irradiation and 1 line of the sporophyte irradiation, suggesting that the materials for the irradiation are applicable for mutant induction. The mutant screening method and the selected mutant candidates would advance the breeding and molecular biology in U. pinnatifida.</p>
収録刊行物
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- CYTOLOGIA
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CYTOLOGIA 86 (4), 291-295, 2021-12-25
公益財団法人 日本メンデル協会
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詳細情報 詳細情報について
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- CRID
- 1390009018098491392
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- NII論文ID
- 130008132480
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- ISSN
- 13487019
- 00114545
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可