Determination of residual antibacterial substances in processed foods manufactured from livestock and marine products using HPLC

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  • Hirata Shotaro
    Division of Hygienic Chemistry, Osaka Institute of Public Health Graduate School of Humanities and Sustainable System Sciences, Osaka Prefecture University
  • Masayama Atsushi
    Division of Hygienic Chemistry, Osaka Institute of Public Health
  • Nakatani Tadashi
    Division of Hygienic Chemistry, Osaka Institute of Public Health
  • Hoshi Hidenobu
    Graduate School of Humanities and Sustainable System Sciences, Osaka Prefecture University
  • Takatori Satoshi
    Division of Hygienic Chemistry, Osaka Institute of Public Health

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Other Title
  • HPLC を用いた畜水産物を主原料とした加工食品中の残留抗菌性物質分析法の検討
  • HPLC オ モチイタ チクスイサンブツ オ シュ ゲンリョウ ト シタ カコウ ショクヒン チュウ ノ ザンリュウ コウキンセイ ブッシツ ブンセキホウ ノ ケントウ

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Abstract

A rapid and easy method for determination of antibacterial substances in processed foods manufactured from livestock and marine products using high performance liquid chromatograph with photodiode array detector (HPLC-PDA) has been developed. Residual antibacterial substances were extracted with a mixture of acetonitrile/methanol and citrate-phosphate buffer. The lipids were removed from the extract by acetonitrile-hexane partition and solid-phase column. After removal of the solvent, the extract was resolved in potassium phosphate solution and analyzed with HPLC-PDA. Recovery tests of 8 major antibacterial substances from processed foods were performed, and all substances exhibited acceptable recoveries (70-120%) with low relative standard deviations. The time for sample preparation with 8 samples to test solutions was approximately 6 hours. This method could be useful for primary screening inspection of residual antibacterial substances in processed foods manufactured from livestock and marine products.

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