A Simple Preparation of Collagen Containing Samples (2)

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  • コラーゲンを含む試料の簡便な調製(2)

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We measured carbon content, ^<14>C content (pM) and δ ^<13>C of 5 collagen containing samples (ivory) which were prepared by a simple preparation method. We chipped off small fragments from 4 samples (IVORY-12,14,15,16) with a knife, washed each fragment samples with acid-alkali-acid method and combusted them to produce CO_2,instead of collagen extraction. About IVORY-13,we crashed it into pieces with a hummer instead of chipping. The chipped samples showed very high carbon contents (nearby the carbon content of collagen), suggesting that immediate decaluciation has occurred. In the next study, we will prepare collagen from these samples to discuss the adequacy of applying this simple preparation method for collagen containing samples that are preserved under good condition.

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