ハムスター BHK21 細胞ならびにそのポリオーマウイルスによるトランスフォーム細胞のForssman抗原反応性に関する研究

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  • Studies on the Reactivity of Forssman Antigen Present in Untransformed BHK-21 Cells and Their Polyoma-transformed Derivatives
  • ハムスターBHK21 サイボウ ナラビニ ソノ ポリオーマ ウイルス ニヨル

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The reactivity of the Forssman antigen on the cell surface was studied in relation to the cell density of culture, cell growth, and cell cycle. In this study an established cell line of baby hamster kidney cells (BHK 21/13) and its polyoma virustransformed derivative (py-BHK) were used. The following results were obtained using an immune adherence (IA) method, in which the reactivity of Forssman antigen was expressed in terms of proportion of IA-positive cells. 1. When the BHK cells were collected from a sparse culture showing no cell-cell contact, 70-85% of the cells were IA-positive. At a density of 3 ×103 cells/cm2 , where the cells commenced to come contact with each other, 60% of the cells were IA-positive. As the cell density increased, the reactivity of the antigen decreased. Only 30-40% of the cells were IA-positive, when a confluent culture was used. It is likely that the cell-cell contact is responsible for the alteration of cell surface leading to a decrease in the reactivity of IA reaction. 2. In the case of py-BHK cells, about 90% of the cells were IA-positive, irrespective of the cell density. 3. The reactivity of the antigen was tested on the BHK cells through their cell cycle, showing that the reactivity increased to 70-90% in a near S phase, while it decreased to about 45% in M and G1 phases. 4. When the BHK cells of a confluent culture were treated with trypsin or neuraminidase, the reactivity of Forssman antigen increased to an extent comparable to that of py-BHK cells. An immunofluorescence examination revealed that all cells were bound with the antibody regardless of their IA reactivity. The results suggest that decrease in the reactivity of Forssman antigen demonstrated by the IA method may be due to a masking phenomenon caused by a renewal and/or rearrangement of surface components.

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